| Cat#: TR8061 | Source#: Rabbit | Type#: Monoclonal | |
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| Applications: WB,IHC-P,IF-P,IF-F,IF-ICC,IP,ELISA |
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Reactivity: Human,Mouse,Rat |
| MW.(KDa): 55,65kDa |
Isotype: IgG, Kappa |
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| secreted phosphoprotein 1(SPP1) Homo sapiens The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011], |
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| Western Blot |

Western blot analysis of lysates from A549 cells, using Osteopontin Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody was used to detect the antibody.
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| Storage |
Store at -20°C. Stable for one year from the date of shipment. |
| Concentration |
1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol. |
| Dilutions |
IHC-P 1:200-1:1000, WB 1:2000-1:10000, IF-P/IF-F/IF-ICC 1:200-1:1000, ELISA 1:5000-1:20000, IP 1:50-1:200
(Optimal dilutions should be determined by the end user)
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| SPP1;BNSP;OPN;PSEC0156;Osteopontin;Bone sialoprotein 1;Nephropontin;Secreted phosphoprotein 1;SPP-1;Urinary stone protein;Uropontin |
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| Size: |
50μL ¥1300 100μL ¥2300 |
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| New Product: |
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