| Cat#： TT1444 | Source#： Rabbit | Type#： Polyclonal | |
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| Applications: WB,IF,ELISA,IHC |
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Reactivity: H,M,R,Mk |
| MW.(KDa): 44 |
Isotype: IgG |
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The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. |
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| Western Blot |
Western Blot analysis of various cells using E2F-4/5 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
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| Immunohistochemistry |
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using E2F4 Antibody. The picture on the right is blocked with the synthesized peptide.
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| Western Blot |
Western blot analysis of lysates from COS7 cells, using E2F4 Antibody. The lane on the right is blocked with the synthesized peptide.
描述：
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| Source |
The antiserum was produced against synthesized peptide derived from human E2F4. AA range:51-100 |
| Specificity |
E2F-4/5 Polyclonal Antibody detects endogenous levels of E2F-4/5 protein |
| Storage |
Store at -20°C. Stable for one year from the date of shipment. |
| Concentration |
1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol. |
| Dilutions |
WB 1:500-2,000; IHC 1:100-300; IF 1:50-200; ELISA 1:40,000
(Optimal dilutions should be determined by the end user)
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| E2F4 ; Transcription factor E2F4 ; E2F-4 ; E2F5 ; Transcription factor E2F5 ; E2F-5 |
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| Size: |
50μL ￥1000
100μL ￥1800 |
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| New Product: |
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| 直接下单: |
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立即下单
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