ATG5 Rabbit Monoclonal Antibody

Key facts:

Catalog NO:	TR8024
Source :	Rabbit
Type:	Monoclonal
Application:	WB,IHC-P,IF-P,IF-F,IF-ICC,IP,ELISA
Reactivity:	Human,Mouse,Rat
Isotype:	IgG, Kappa
MW(KDa):	55kDa
Alternative Names:	Autophagy protein 5 (APG5-like;Apoptosis-specific protein);APG5L;ASP

Specifications:

Specificity

Endogenous

Storage

Store at -20°C. Stable for one year from the date of shipment.

Concentration

1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.

Dilutions

IHC-P 1:200-1:1000, WB 1:2000-1:10000, IF-P/IF-F/IF-ICC 1:200-1:1000, ELISA 1:5000-1:20000, IP 1:50-1:200

(Optimal dilutions should be determined by the end user)

Background:

The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Sep 2015],

Applications:

Western Blot

Western blot analysis of lysates from U-87 MG cells, using ATG5 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody was used to detect the antibody.

Immunohistochemistry

Immunohistochemical analysis of paraffin-embedded Human colon tissue. 1, primary Antibody was diluted at 1:200(4℃,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98℃,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).

Contact information:

Company:	BEI JING TDY BIOTECH CO.,LTD.
Orders:	TDYbiotech@163.com
Website:	http://www.tdybio.com
Telephone:	010-80117836
Address:	Zhen Xin Road 36.Changping Distict,Beijing

Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig

Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation

Please note: All products are"FOR RESEARCH USE ONLY.NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES".