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E2F-4/5 Rabbit pAb
Key facts:
Catalog NO:TT1444
Source :Rabbit
Type:Polyclonal
Application:WB,IF,ELISA,IHC
Reactivity:H,M,R,Mk
Isotype:IgG
MW(KDa):44
Alternative Names:E2F4 ; Transcription factor E2F4 ; E2F-4 ; E2F5 ; Transcription factor E2F5 ; E2F-5
Specifications:
Source The antiserum was produced against synthesized peptide derived from human E2F4. AA range:51-100

 

Specificity E2F-4/5 Polyclonal Antibody detects endogenous levels of E2F-4/5 protein

 

Storage Store at -20°C. Stable for one year from the date of shipment.

 

Concentration 1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.

 

Dilutions

 WB 1:500-2,000; IHC 1:100-300; IF 1:50-200; ELISA 1:40,000 

(Optimal dilutions should be determined by the end user)
Background:
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two.
Applications:
Western Blot                                 

Western Blot analysis of various cells using E2F-4/5 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using E2F4 Antibody. The picture on the right is blocked with the synthesized peptide.
Western Blot                            

Western blot analysis of lysates from COS7 cells, using E2F4 Antibody. The lane on the right is blocked with the synthesized peptide.
描述：
Contact information:
Company:BEI JING TDY BIOTECH CO.,LTD.
Orders:TDYbiotech@163.com
Website:http://www.tdybio.com
Telephone:010-80117836
Address:Zhen Xin Road 28.Changping Distict,Beijing
Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig
Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation
Please note: All products are"FOR RESEARCH USE ONLY.NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES".