010-80117836
276385203@qq.com |
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| β-tubulin Mouse mAb(Mix-mA™) |
| Beta 4 tubulin, TBB5, TUBB2, TUBB2A, tubulin beta 2A, beta tubulin, tubulin beta chain |
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| Cat#： TDY167 | Source： Mouse | Type： Monoclonal | |
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| Applications: WB, IHC, IP |
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Reactivity: H, R, M, Mk, Dg, C, Hm, Rb, Sh |
| MW.(KDa): 55 |
Isotype: IgG1 |
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Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation
Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig
Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Tubulin may not be stable in certain cells. For example, expression of β-Tubulin in adipose tissue is very low and therefore β-Tubulin should not be used as loading control for these tissues.
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| Western Blot |
Western blot analysis of 1) Hela ,2) Mouse Kidney tissue, 3) Rat Heart tissue with TDY167 diluted at 1:5,000.
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| IP |
1、Input: Mouse Brain Tissue Lysate
2、IP product: IP dilute 1:200
Western blot analysis: primary antibody : TDY167 1:5,000
Secondary antibody: Goat anti-Mouse IgG, Light chain specific (S003), 1:5,000
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| Source |
A Mouse Mixture monoclonal antibody is prepared by immunizing recombinant protein.
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| Specificity |
The β-tubulin Mouse mAb(Mix-mA™) can recognize endogenous β-tubulin proteins.
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| Storage |
Store at -20°C. Stable for one year from the date of shipment.
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| Concentration |
1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.
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| Dilutions |
WB 1:5,000-10,000 IHC 1:200 IP 1:200
(Optimal dilutions should be determined by the end user)
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Beta 4 tubulin, TBB5, TUBB2, TUBB2A, tubulin beta 2A, beta tubulin, tubulin beta chain |
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| Size: |
TDY167C-50μL ￥1000
TDY167F-100μL ￥1800 |
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