010-80117836
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β-actin Rabbit pAb(A284) |
actin antibody, b-actin antibody, beta-actin antibody |
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Cat#: TDY576 | Source: Rabbit | Type: Polyclonal | |
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Applications: WB, IHC |
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Reactivity: H, R, M |
MW.(KDa): 43 |
Isotype: IgG |
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Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation
Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig
β-actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, antibodies against β-actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-actin may not be stable in certain cells. For example, expression of β-actin in adipose tissue is very low and therefore β-actin should not be used as loading control for these tissues. |
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Western Blot |
Western blot analysis of 1) Hela Cell Lysate, 2) C2C12 Cell Lysate, 3) PC12 Cell Lysate, 4) Rat Brain Tissue Lysate using β-actin (TDY576)Rabbit pAb diluted at 1:5000.
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IHC |
Immunohistochemical analysis of paraffin-embedded Human Heart Tissue using β-actin (TDY576)Rabbit pAb diluted at 1:200.
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IHC |
Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using β-actin (TDY576)Rabbit pAb diluted at 1:200.
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Source |
Rabbit Polyclonal antibody is prepared by immunizing synthetic peptide coupled to KLH.
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Specificity |
The β-actin Rabbit Polyclonal antibody can detect endogenous β-actin proteins.
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Storage |
Store at -20°C. Stable for one year from the date of shipment.
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Concentration |
1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.
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Dilutions |
WB 1:2,000-5,000 IHC 1:100-200
(Optimal dilutions should be determined by the end user)
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actin antibody, b-actin antibody, beta-actin antibody |
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Size: |
TDY576C-50μL ¥1000 TDY576F-100μL ¥1800 |
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New Product: |
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