010-80117836
276385203@qq.com |
| |
|
|
|
| PCNA Mouse mAb(12D10) |
| Proliferating Cell Nuclear Antigen antibody, DNA polymerase delta auxiliary protein antibody, PCNAR antibody |
|
| |
| Cat#： TDY044 | Source： Mouse | Type： Monoclonal | |
 |
| Applications: WB, IHC |
|
Reactivity: H, R, M |
| MW.(KDa): 29 |
Isotype: IgG1 |
|
Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation
Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig
PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. It is found in the nucleus and is a cofactor of DNA polymerase delta. PCNA acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6 dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
|
|
|
|
| Western Blot |
Western blot analysis of Hela (1), Rat brain (2), NIH 3T3 (3), 293T(4) with PCNA mouse mAb(12D10) diluted at 1:5000.
|
| Immunohistochemistry |
IHC staining of Human breast cancer tissue paraffin-embedded with PCNA mouse mAb（12D10）diluted at 1:200
|
|
|
| |
|
|
| Source |
Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH.
|
| Specificity |
The PCNA mouse monoclonal antibody can detect endogenous PCNA proteins.
|
| Storage |
Store at -20°C. Stable for one year from the date of shipment.
|
| Concentration |
1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.
|
| Dilutions |
WB 1:5,000 IHC 1:200
(Optimal dilutions should be determined by the end user)
|
|
|
| |
|
Proliferating Cell Nuclear Antigen antibody, DNA polymerase delta auxiliary protein antibody, PCNAR antibody |
| |
|
|
|
|
| Size: |
TDY044C-50μL ￥800
TDY044F-100μL ￥1500 |
|
| New Product: |
|
|
|
|
| |
|
