010-80117836
276385203@qq.com |
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| β-actin Rabbit pAb |
| actin antibody, b-actin antibody, beta-actin antibody |
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| Cat#： TDY051 | Source： Rabbit | Type： Polyclonal | |
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| Applications: WB, IHC, IF |
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Reactivity: H, R, M, Mk, Ch, Hm, Sh，Pg，Xenopus |
| MW.(KDa): 43 |
Isotype: IgG |
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Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation
Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig
β-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against β-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Actin may not be stable in certain cells. For example, expression of β-Actin in adipose tissue is very low and therefore β-Actin should not be used as loading control for these tissues.
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| Western Blot |
Western blot analysis of HepG2 (1) , Rat liver (2) ,Mouse kidney (3) , Rabbit testic(4), Sheep lung(5), 293T(6) with β-Actin rabbit polyclonal antibody diluted at 1:5000.
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| Immunohistochemistry |
IHC staining of Human ovary tissue with β-Actin pAb diluted at 1:200.
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| IF |
IF analysis of Hela with TDY051 diluted at 1:500.
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| Source |
Rabbit polyclonal antibody is prepared by immunizing recombinant protein.
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| Specificity |
The β-actin rabbit polyclonal antibody can detect endogenous β-actin proteins.
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| Storage |
Store at -20°C. Stable for one year from the date of shipment.
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| Concentration |
1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.
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| Dilutions |
WB 1:3,000 IHC 1:200 IF 1:200
(Optimal dilutions should be determined by the end user)
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actin antibody, b-actin antibody, beta-actin antibody |
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| Size: |
TDY051C-50μL ￥800
TDY051F-100μL ￥1500 |
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| New Product: |
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