010-80117836
276385203@qq.com |
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| Flag-Tag Mouse mAb(2C5) |
| DDDDK Tag antibody, DYKDDDDK Tag antibody |
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| Cat#： TDY001 | Source： Mouse | Type： Monoclonal | |
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| Applications: WB, IP, IF |
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Reactivity: N/A |
| MW.(KDa): N/A |
Isotype: IgG1 |
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Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation
Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig
The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence. |
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| IP |
IP antibody use：5ug Flag Mouse IgG1 per ml Lysate，WB 1:5000
1、untransfected 293 cell lysate
2、transfected 293 cell lysate with Flag-tag fusion protein
3、IP (transfected 293+ normal Mouse IgG+Protein G agarose)
4、IP (transfected 293+anti- Flag mAb+ Protein G agarose)
5、IP (transfected 293+Protein G)
6、Recombinant protein (E.coli)
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| Western Blot |
1ug Flag fusion protein+ Primary antibody dilution at 1:10,000.
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| IF |
IF analysis of 293 cells transfected with a Flag-tag protein, using TDYbio anti-FlagTag Mouse mAb at a 1:2000 dilution (blue DAPI, red anti-Flag)
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| Source |
Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH.
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| Specificity |
The Flag tag antibody can recognize C-terminal, internal, and N-terminal Flag-tag fusion proteins.
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| Storage |
Store at -20°C. Stable for one year from the date of shipment.
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| Concentration |
1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.
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| Dilutions |
WB 1:5,000 IP 1:200 IF 1:2,000
(Optimal dilutions should be determined by the end user)
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DDDDK Tag antibody, DYKDDDDK Tag antibody |
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| Size: |
TDY001C-50μL ￥800
TDY001F-100μL ￥1500 |
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