010-80117836
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| NFκB p65 Mouse mAb(14H2) |
| Nuclear Factor NF Kappa B p65 Subunit antibody, p65 NF kappaB antibody, Transcription Factor p65 antibody |
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| Cat#： TDY076 | Source： Mouse | Type： Monoclonal | |
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| Applications: WB, IHC, IP |
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Reactivity: H,M,R |
| MW.(KDa): 65 |
Isotype: IgG1 |
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Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation
Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig
NFkB p65 is ubiquitinated leading to its proteosomal degradation, which is required for termination of the NFkB response. Phosphorylation of NFkB p65 on S536 stimulates acetylation of K310 by CBP, enhancing transcriptional activity. NFkB p65 is also acetylated at K122, enhancing DNA binding and impairing the interaction with NFKBIA. The protein is deacetylated by HDAC3. Invasion of a host by a pathogen is frequently associated with the activation of NF-kB, which coordinates various aspects of immune function required for resistance to infection.
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| Western Blot |
Western blot analysis of 1) Hela, 2)293T,3)HepG with NFκB p65 mAb diluted at 1:2,000.
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| Immunohistochemistry |
IHC staining of mouse hippocampus tissue with NFκB p65 mouse mAb(14H2) diluted at 1:200.
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| Western Blot |
Western blot analysis of 1) Mouse Heart, 2)Rat Heart,3)Rat Liver, 4) Rat Spleen with NFκB p65 mAb diluted at 1:3,000.
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| IP |
1、Input: Hela Cell Lysate
2、IP product: IP dilute 1:200
Western blot analysis: primary antibody : TDY076 1:2,000
Secondary antibody: Goat anti-Mouse IgG, Light chain specific(S003), 1:5,000
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| Source |
Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH.
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| Specificity |
The NFκB p65 mouse monoclonal antibody can detect endogenous NFκB p65 proteins.
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| Storage |
Store at -20°C. Stable for one year from the date of shipment.
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| Concentration |
1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.
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| Dilutions |
WB 1:1,000-3,000 IHC 1:200 IP 1:200
(Optimal dilutions should be determined by the end user)
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Nuclear Factor NF Kappa B p65 Subunit antibody, p65 NF kappaB antibody, Transcription Factor p65 antibody |
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| Size: |
TDY076C-50μL ￥1000
TDY076F-100μL ￥1800 |
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| New Product: |
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