010-80117836 
          276385203@qq.com  | 
       
      
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              | CK7 Mouse mAb(12D7) | 
             
            
              | Cytokeratin 7 antibody, Keratin-7 antibody, Type II mesothelial keratin K7 antibody | 
             
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                  | Cat#: TDY077   |   Source: Mouse   |   Type: Monoclonal   |  | 
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                        | Applications: WB, IHC, IP | 
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                        Reactivity: H, M,R  | 
                       
                      
                        | MW.(KDa): 54  | 
                        Isotype: IgG1  | 
                       
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                    Applications:          WB-Western blot     IHC-Immunochemistry       IF-Immunofluorescence     IP-Immunoprecipitation     ChIP-Chormatin Immunoprecipitation 
Reactivity:    H-Human  R-Rat  M-Mouse  Mk-Monkey  Dg-Dog  Ch-Chicken  Hm-Hamster  Rb-Rabbit  Sh-Sheep  Pg-Pig 
  
Cytokeratin 7 is a 54kD intermediate filament protein found in a variety of glandularepithelia. Cytokeratin 7 has been found in columnar and glandular epithelium of the lung, cervix, breast, bile ducts and larger collecting ducts of the kidney. It is present in the transitional epithelium of bladder as well as ovarian and lungepithelia, and occasionally staining of blood vessel cell walls, particularly endothelial cells, may be observed. However, Cytokeratin 7 is not expressed by epithelial cells of the gastrointestinal tract, colon or prostate. Keratin 7 is often co expressed with keratin 19.  
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                        | Western Blot | 
                        
                            
                        Western blot analysis of 1) Hela, 2)Mouse Kidney, 3) Mouse Brain with CK7 mAb diluted at 1:2,000.  
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                        | Immunohistochemistry | 
                        
                            
                        IHC staining of human lung cancer tissue with CK7 mouse mAb(12D7) diluted at 1:200.  
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                        | IP | 
                        
                             
                        1、Input: Hela Cell Lysate 
                        2、IP product: IP dilute 1:200 
                        Western blot analysis: primary antibody : TDY077     1:1,000 
                        Secondary antibody: Goat anti-Mouse IgG, Light chain specific(S003), 1:5,000  
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                        | Source | 
                        
                         Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH. 
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                        | Specificity | 
                        
                         The CK7 mouse monoclonal antibody can detect endogenous CK7 proteins. 
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                        | Storage | 
                        
                         Store at -20°C. Stable for one year from the date of shipment.  
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                        | Concentration | 
                        
                         1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol. 
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                        | Dilutions | 
                        
                         WB 1:1,000-2,000 IHC 1:200 IP 1:200 
                        (Optimal dilutions should be determined by the end user) 
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                                  Cytokeratin 7 antibody, Keratin-7 antibody, Type II mesothelial keratin K7 antibody  | 
                                 
                                
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                  |   Size: | 
                 
                
                  TDY077C-50μL ¥1000 TDY077F-100μL ¥1800 | 
                 
                
                  
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