010-80117836 
          276385203@qq.com  | 
       
      
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              | Caspase 9 Mouse mAb(TDY3-20) | 
             
            
              | APAF 3 antibody, Apoptosis related cysteine peptidase antibody, Apoptotic protease-activating factor 3 antibody, CASP  | 
             
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                  | Cat#: TDY102   |   Source: Mouse   |   Type: Monoclonal   |  | 
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                        | Applications: WB, IP, IF | 
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                        Reactivity: H, R, M  | 
                       
                      
                        | MW.(KDa): 47KD(H), 51KD(R, M)  | 
                        Isotype: IgG1  | 
                       
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                    Applications:          WB-Western blot     IHC-Immunochemistry       IF-Immunofluorescence     IP-Immunoprecipitation     ChIP-Chormatin Immunoprecipitation 
Reactivity:    H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig 
  
Caspase-9 is an initiator caspase, encoded by the CASP9 gene.Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).   | 
                   
                 
							  
            
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                        | Western Blot | 
                        
                            
                        Western blot analysis of Hela with Caspase 9 (TDY3-20) Mouse mAb diluted at  
                        1)1:2,000  
                        2)1:5,000  
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                        | Western Blot | 
                        
                            
                        Western blot analysis of 1)Jurkat, 2) HepG2, 3) Mouse Heart, 4) Rat Heart with Caspase 9 (TDY3-20) Mouse mAb diluted at 1:3,000 
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                        | IP | 
                        
                             
                        1、Input: Hela Cell Lysate 
                        2、IP product: IP dilute 1:200 
                        Western blot analysis: primary antibody : TDY102   1:1,000 
                        Secondary antibody: Goat anti-Mouse IgG, Light chain specific(S003), 1:5,000  
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                        | IF | 
                        
                            
                        IF analysis of Hela with TDY102(Left) and DAPI (Right) diluted at 1:100. 
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                        | Source | 
                        
                         Mouse monoclonal antibody is prepared by immunizing recombinant protein. 
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                        | Specificity | 
                        
                         The Caspase 9 antibody can recognize endogenous Caspase 9 proteins. 
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                        | Storage | 
                        
                         Store at -20°C. Stable for one year from the date of shipment.  
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                        | Concentration | 
                        
                         1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol. 
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                        | Dilutions | 
                        
                         WB 1:1,000-5,000 IP 1:200 IF 1:100-200 
                        (Optimal dilutions should be determined by the end user) 
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                                  APAF 3 antibody, Apoptosis related cysteine peptidase antibody, Apoptotic protease-activating factor 3 antibody, CASP 9 antibody  | 
                                 
                                
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                  |   Size: | 
                 
                
                  TDY102C-50μL ¥1000 TDY102F-100μL ¥1800 | 
                 
                
                  
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                  |   New Product: | 
                 
                
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