010-80117836
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Caspase 9 Mouse mAb(TDY3-20) |
APAF 3 antibody, Apoptosis related cysteine peptidase antibody, Apoptotic protease-activating factor 3 antibody, CASP |
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Cat#: TDY102 | Source: Mouse | Type: Monoclonal | |
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Applications: WB, IP, IF |
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Reactivity: H, R, M |
MW.(KDa): 47KD(H), 51KD(R, M) |
Isotype: IgG1 |
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Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation
Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig
Caspase-9 is an initiator caspase, encoded by the CASP9 gene.Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). |
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Western Blot |
Western blot analysis of Hela with Caspase 9 (TDY3-20) Mouse mAb diluted at
1)1:2,000
2)1:5,000
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Western Blot |
Western blot analysis of 1)Jurkat, 2) HepG2, 3) Mouse Heart, 4) Rat Heart with Caspase 9 (TDY3-20) Mouse mAb diluted at 1:3,000
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IP |
1、Input: Hela Cell Lysate
2、IP product: IP dilute 1:200
Western blot analysis: primary antibody : TDY102 1:1,000
Secondary antibody: Goat anti-Mouse IgG, Light chain specific(S003), 1:5,000
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IF |
IF analysis of Hela with TDY102(Left) and DAPI (Right) diluted at 1:100.
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Source |
Mouse monoclonal antibody is prepared by immunizing recombinant protein.
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Specificity |
The Caspase 9 antibody can recognize endogenous Caspase 9 proteins.
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Storage |
Store at -20°C. Stable for one year from the date of shipment.
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Concentration |
1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.
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Dilutions |
WB 1:1,000-5,000 IP 1:200 IF 1:100-200
(Optimal dilutions should be determined by the end user)
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APAF 3 antibody, Apoptosis related cysteine peptidase antibody, Apoptotic protease-activating factor 3 antibody, CASP 9 antibody |
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Size: |
TDY102C-50μL ¥1000 TDY102F-100μL ¥1800 |
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New Product: |
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