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Histone H3 (di methyl K27) Mouse mAb(3B12) |
H3 histone antibody, HIST1H3A antibody, Histone cluster 1, H3a antibody |
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Cat#: TDY128 | Source: Mouse | Type: Monoclonal | |
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Applications: WB,IHC,IP |
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Reactivity: H, R, M |
MW.(KDa): 15 |
Isotype: IgG1 |
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Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation
Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
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Western Blot |
Western blot analysis of Hela withHistone H3 (di methyl K27) (1E10) Mouse MAb diluted at
1) 1:1,000
2) 1:3,000
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IP |
1、Input: Hela Cell Lysate
2、IP product: IP dilute 1:200
Western blot analysis: primary antibody : TDY128 1:2,000
Secondary antibody: Goat anti-Mouse IgG(H+L) (S001) 1:10,000
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IHC |
Immunohistochemical analysis of paraffin-embedded Human Skin using Histone H3(di methyl K27)(TDY128)Mouse mAb diluted at 1:500.
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Source |
Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH.
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Specificity |
The Histone H3 (di methyl K27) antibody can recognize endogenous Histone H3 (di methyl K27) proteins.
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Storage |
Store at -20°C. Stable for one year from the date of shipment.
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Concentration |
1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.
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Dilutions |
WB 1:1,000-3,000 IP 1:200 IHC 1:200-500
(Optimal dilutions should be determined by the end user)
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H3 histone antibody, HIST1H3A antibody, Histone cluster 1, H3a antibody |
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Size: |
TDY128C-50μL ¥1000 TDY128F-100μL ¥1800 |
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New Product: |
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