010-80117836
276385203@qq.com |
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| NFκB p65 Mouse mAb(5G6) |
| Nuclear Factor NF Kappa B p65 Subunit antibody, p65 NF kappaB antibody, Transcription Factor p65 antibody |
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| Cat#： TDY138 | Source： Mouse | Type： Monoclonal | |
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| Applications: WB, IP, IF, IHC |
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Reactivity: H, R, M |
| MW.(KDa): 65 |
Isotype: IgG1 |
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Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation
Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig
NFkB p65 is ubiquitinated leading to its proteosomal degradation, which is required for termination of the NFkB response. Phosphorylation of NFkB p65 on S536 stimulates acetylation of K310 by CBP, enhancing transcriptional activity. NFkB p65 is also acetylated at K122, enhancing DNA binding and impairing the interaction with NFKBIA. The protein is deacetylated by HDAC3. Invasion of a host by a pathogen is frequently associated with the activation of NF-kB, which coordinates various aspects of immune function required for resistance to infection. |
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| Western Blot |
Western blot analysis of 1) Hela, 2) Rat Heart Tissue, 3) Mouse Spleen Tissue with TDY138 diluted at 1:2000.
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| IP |
1、Input: Hela Cell Lysate
2、IP product: IP dilute 1:200
Western blot analysis: primary antibody : TDY138 1:2,000
Secondary antibody: Goat anti-Mouse IgG, Light chain specific(S003), 1:5,000
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| IF |
IF analysis of Hela with TDY138(Left) and DAPI (Right) diluted at 1:100.
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| IHC |
Immunohistochemical analysis of paraffin-embedded Rat Brain Tissue using NFkB p65 （TDY138）Mouse mAbdiluted at 1:500.
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| Source |
Mouse monoclonal antibody is prepared by immunizing recombinant protein.
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| Specificity |
The NFκB p65 mouse monoclonal antibody can detect endogenous NFκB p65 proteins.
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| Storage |
Store at -20°C. Stable for one year from the date of shipment.
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| Concentration |
1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.
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| Dilutions |
WB 1:500-2,000 IP 1:200 IF 1:100-200 IHC 1:200-500
(Optimal dilutions should be determined by the end user)
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Nuclear Factor NF Kappa B p65 Subunit antibody, p65 NF kappaB antibody, Transcription Factor p65 antibody |
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| Size: |
TDY138C-50μL ￥1000
TDY138F-100μL ￥1800 |
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| New Product: |
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