010-80117836
276385203@qq.com |
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| His-Tag Mouse mAb(M1) |
| 6 His antibody, 6*His |
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| Cat#： TDY157 | Source： Mouse | Type： Monoclonal | |
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| Applications: WB, IP |
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Reactivity: N/A |
| MW.(KDa): N/A |
Isotype: IgG1 |
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Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation
Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells. |
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| Western Blot |
Western blot analysis of His-Tag recombinant protein with TDY157 diluted at 1:5,000
1) His-Tag transfected 293T
2) His-MBP recombinant protein
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| Source |
Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH.
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| Specificity |
The His tag antibody can recognize C-terminal, internal, and N-terminal His-tag fusion proteins.
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| Storage |
Store at -20°C. Stable for one year from the date of shipment.
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| Concentration |
1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.
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| Dilutions |
WB 1:3,000-5,000 IP 1:500
(Optimal dilutions should be determined by the end user)
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| Size: |
TDY157C-50μL ￥800
TDY157F-100μL ￥1500 |
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| New Product: |
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