| Cat#： TDY004 | Source： Mouse | Type： Monoclonal | |
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| Applications: WB, IP, IF |
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Reactivity: N/A |
| MW.(KDa): N/A |
Isotype: IgG1 |
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Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation
Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells. |
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| IP |
IP antibody use：5ug His Mouse IgG1 per ml Lysate，WB 1:3000
1、untransfected 293 cell lysate
2、transfected 293 cell lysate with His-tag fusion protein
3、IP (untransfected 293+anti-His mAb+ Protein G agarose)
4、IP (transfected 293+ normal Mouse IgG+Protein G agarose)
5、IP (transfected 293+anti-His mAb+ Protein G agarose)
6、Recombinant protein (E.coli)
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| IF |
IF analysis of 293 cells transfected with a His-tag protein, using TDYbio anti-HisTag Mouse mAb at a 1:1000 dilution (blue DAPI, red anti-His)
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| Western Blot |
2ug His fusion protein+ Primary antibody dilution at
1、1:5,000
2、1:10,000
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| Western Blot |
1、Multi-Tag Recombinant protein
2、293T transfected with His recombinant protein
3、Insect cell trandfected with His recombinant protein
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| Source |
Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH.
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| Specificity |
The His tag antibody can recognize C-terminal, internal, and N-terminal His-tag fusion proteins.
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| Storage |
Store at -20°C. Stable for one year from the date of shipment.
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| Concentration |
1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.
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| Dilutions |
WB 1:5,000 IP 1:200 IF 1:1,000
(Optimal dilutions should be determined by the end user)
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| Size: |
TDY004C-50μL ￥800
TDY004C-100μL ￥1500 |
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| New Product: |
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