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内参蛋白抗体
标签蛋白抗体
荧光蛋白抗体
Epi-XAb™抗体
常用蛋白抗体
ImmunoWay组织病理学抗体
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VSV-G-Tag Mouse mAb(8D6)
VSV tag antibody, VSVG tag antibody
 
Cat#: TDY006   |   Source: Mouse   |   Type: Monoclonal   |
Applications: WB, IP, IF   Reactivity: N/A
MW.(KDa): N/A Isotype: IgG1

Applications:          WB-Western blot     IHC-Immunochemistry       IF-Immunofluorescence     IP-Immunoprecipitation     ChIP-Chormatin Immunoprecipitation

Reactivity:    H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig
 
Vesicular stomatitis virus (VSV), an enveloped RNA virus from the Rhabdoviridae family, is released from the plasma membrane of host cells by a process called budding. The fusiogenic envelope G glycoprotein of the vesicular stomatitis virus (VSV-G) that has been used to pseudotype retrovirus and lentivirus vectors can be used alone as an efficient vehicle for gene transfer. VSV-G protein is secreted into the culture medium as sendimentable vesicles from cells transfected with a VSV-G expression plasmid in the absence of other viral components. The VSV-G vesicles in the conditioned medium can be partially purified by pelleting through sucrose cushion ultracentrifugation. 
Applications Specifications Alternative Names

IP

 

IP antibody use5ug VSV-G Mouse IgG1 per ml LysateWB 1:5000

1untransfected 293 cell lysate

2transfected 293 cell lysate with VSV-G-tag fusion protein

3IP(untransfted 293+anti-VSV-G mAb+Protein G agarose)

4IP (transfected 293+ normal Mouse IgG+Protein G agarose)

5IP (transfected 293+anti-VSV-G mAb+ Protein G agarose)

6IP (transfected 293+Protein G)

7Recombinant protein (E.coli)



IF

 

IF analysis of 293T cells transfected with a VSV-G-tagged protein, using TDYbio VSV-G-Tag (8D6) Mouse mAb at a 1:2000 dilution (blue DAPI ,red anti-VSV-G)

 

Western Blot

 

1ug VSV-G fusion protein+ Primary antibody dilution at

11:5,000

21:10,000

 

 
  Size:
TDY006C-50μL ¥800
TDY006F-100μL ¥1500

  New Product:
ImmunoWay组织病理学抗体
 
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