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HSP70 Mouse mAb(3G10)
heat shock protein 70 antibody
 
Cat#: TDY062   |   Source: Mouse   |   Type: Monoclonal   |
Applications: WB, IF, IHC   Reactivity: H, M, R,Pg
MW.(KDa): 70 Isotype: IgG1

Applications:          WB-Western blot     IHC-Immunochemistry       IF-Immunofluorescence     IP-Immunoprecipitation     ChIP-Chormatin Immunoprecipitation

Reactivity:    H-Human  R-Rat  M-Mouse  Mk-Monkey  Dg-Dog  Ch-Chicken  Hm-Hamster  Rb-Rabbit  Sh-Sheep  Pg-Pig

 

The 70 kilodalton heat shock proteins (Hsp70s) are a family of ubiquitously expressed heat shock proteins. Proteins with similar structure exist in virtually all living organisms. The Hsp70s are an important part of the cell's machinery for protein folding, and help to protect cells from stress. Hsp70 is usually in an ATP bound state. Hsp70 by itself is characterized by a very weak ATPase activity, such that spontaneous hydrolysis will not occur for many minutes. As newly synthesized proteins emerge from the ribosomes, the substrate binding domain of Hsp70 recognizes sequences of hydrophobic amino acid residues, and interacts with them. This spontaneous interaction is reversible, and in the ATP bound state Hsp70 may relatively freely bind and release peptides. However, the presence of a peptide in the binding domain stimulates the ATPase activity of Hsp70, increasing its normally slow rate of ATP hydrolysis. 

 

Applications Specifications Alternative Names

Western Blot

 

Western blot analysis of 1) Hela, 2) Mouse Brain, with HSP70 mAb diluted at 1:2,000.



Western Blot

 

Western blot analysis of Pig Skeletal Muscle with HSP70 mAb diluted at 1:2,000.

 

IF

 

IF analysis of Hela with TDY062(Left) and DAPI (Right) diluted at 1:100.

IHC

 

Immunohistochemical analysis of paraffin-embedded Human Lung caricnoma using HSP70 TDY062Mouse mAb diluted at 1:500.

 

 
  Size:
TDY062C-50μL ¥1000
TDY062F-100μL ¥1800

  New Product:
ImmunoWay组织病理学抗体
 
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