010-80117836
276385203@qq.com |
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| eIF4A1 Mouse mAb(M8) |
| DDX2, eIF 4A I, Eukaryotic initiation factor 4A-1, ATP-dependent RNA helicase eIF4A-1,DDX2A |
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| Cat#： TDY164 | Source： Mouse | Type： Monoclonal | |
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| Applications: WB, IF, IHC |
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Reactivity: H, R, M |
| MW.(KDa): 48 |
Isotype: IgG1 |
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Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation
Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig
The eukaryotic initiation factor-4A (eIF4A) family consists of 3 closely related proteins EIF4A1, EIF4A2, and EIF4A3. These factors are required for the binding of mRNA to 40S ribosomal subunits. Eukaryotic initiation factor (eIF) complex 2 forms a ternary complex with GTP and the initiator Met-tRNA – this process is regulated by guanine nucleotide exchange and phosphorylation and serves as the main regulatory element of the bottleneck of protein expression. |
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| Western Blot |
Western blot analysis of 1) 293T ,2) Hela, 3) HepG2, 4) Mouse Brain tissue, 5) Rat Brain tissue with TDY164 diluted at 1:2,000.
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| IF |
IF analysis of Hela with TDY164(Left) and DAPI (Right) diluted at 1:100.
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| IHC |
Immunohistochemical analysis of paraffin-embedded Human Lung Caricnoma using eIF4A1（TDY164）Mouse mAb diluted at 1:200.
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| Source |
Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH.
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| Specificity |
The eIF4A1 antibody can recognize endogenous eIF4A1 proteins.
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| Storage |
Store at -20°C. Stable for one year from the date of shipment.
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| Concentration |
1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.
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| Dilutions |
WB 1:1,000-3,000 IF 1:100-200 IHC 1:100-200
(Optimal dilutions should be determined by the end user)
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DDX2, eIF 4A I, Eukaryotic initiation factor 4A-1, ATP-dependent RNA helicase eIF4A-1,DDX2A |
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| Size: |
TDY164C-50μL ￥1000
TDY164F-100μL ￥1800 |
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| New Product: |
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