010-80117836
276385203@qq.com |
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| β-actin Mouse mAb(5B7) |
| actin antibody, b-actin antibody, beta-actin antibody |
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| Cat#： TDY041 | Source： Mouse | Type： Monoclonal | |
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| Applications: WB, IHC, IF |
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Reactivity: H, R, M, Mk, Dg, Ch, Hm, Rb, Pg, Sh, Insect |
| MW.(KDa): 43 |
Isotype: IgG1 |
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Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation
Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig
β-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against β-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Actin may not be stable in certain cells. For example, expression of β-Actin in adipose tissue is very low and therefore β-Actin should not be used as loading control for these tissues.
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| Western Blot |
Western blot analysis of Hela (1) Rat brain (2)Mouse brain (3) Chicken lung (4) and Rabbit testis (5)with β-Actin mouse mAb(5B7) diluted at 1:10,000.
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| Immunohistochemistry |
IHC Staining of Human ovary tissue with β-Actin mouse mAb(5B7) diluted at 1:200
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| Western Blot |
Western blot analysis of Hamster Kidney with β-Actin mouse mAb(5B7) diluted at 1:10,000.
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| Western Blot |
Western blot analysis of 293T(1) , NIH3T3(2) with β-Actin mouse mAb(5B7) diluted at 1:10,000.
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| Source |
Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH.
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| Specificity |
The β-actin mouse monoclonal antibody can detect endogenous β-actin proteins.
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| Storage |
Store at -20°C. Stable for one year from the date of shipment.
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| Concentration |
1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.
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| Dilutions |
WB 1:5,000 IHC 1:200 IF 1:200
(Optimal dilutions should be determined by the end user)
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actin antibody, b-actin antibody, beta-actin antibody |
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| Size: |
TDY041C-50μL ￥800
TDY041F-100μL ￥1500 |
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| New Product: |
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