010-80117836
276385203@qq.com |
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| EGFR Mouse mAb(M6) |
| Epidermal growth factor receptor antibody, erbb 1 antibody, Receptor tyrosine protein kinase ErbB 1 antibody |
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| Cat#： TDY162 | Source： Mouse | Type： Monoclonal | |
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| Applications: WB, IP, IF, IHC |
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Reactivity: H |
| MW.(KDa): 170 |
Isotype: IgG1 |
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Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation
Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig
The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. |
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| Western Blot |
Western blot analysis of 1) A431 ,2) Hela, with TDY162 diluted at 1:2,000.
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| IP |
1、Input: Hela Cell Lysate
2、IP product: IP dilute 1:200
Western blot analysis: primary antibody : TDY162 1:1,000
Secondary antibody: Goat anti-Mouse IgG, Heavy Chain Specific(S003), 1:5,000
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| IF |
IF analysis of Hela with TDY162(Left) and DAPI (Right) diluted at 1:100.
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| Source |
Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH.
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| Specificity |
The EGFR antibody can recognize endogenous EGFR proteins.
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| Storage |
Store at -20°C. Stable for one year from the date of shipment.
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| Concentration |
1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.
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| Dilutions |
WB 1:1,000-3,000 IP 1:200 IF 1:100-200
(Optimal dilutions should be determined by the end user)
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Epidermal growth factor receptor antibody, erbb 1 antibody, Receptor tyrosine protein kinase ErbB 1 antibody |
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| Size: |
TDY162C-50μL ￥1000
TDY162F-100μL ￥1800 |
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| New Product: |
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